Cryoprotective Agents

03/01/2010 - "The aim of this study was to evaluate the cryoprotective effect of different freezing extenders against cryopreservation injuries on Iberian boar sperm. "
01/01/2020 - "However, high concentrations (up to ~ 6-8 M) of cryoprotective agents (CPAs) used in stem cell induce osmotic and metabolic injuries. "
06/01/2017 - "The major challenges to vitrification are devitrification and recrystallization during the warming process, and high concentrations of cryoprotective agents (CPAs) induced metabolic and osmotic injuries. "
03/01/2016 - "We designed a low-cost, easy-to-use, microfluidics-based microperfusion chamber to investigate the osmotic responses of human umbilical vein endothelial cells (HUVECs) at different temperatures, and then optimized the protocols for using cryoprotective agents (CPAs) to minimize osmotic injuries and improve processes before freezing and after thawing. "
01/01/2012 - "In the following paper, the introduction of cryoprotective agents (CPAs) used during cryopreservation of cells in order to protect them from freezing injuries and increase viability post thaw is described. "

11/01/2014 - "However, few studies have investigated the involvement of IGF-I during states of extreme shock, such as those induced by potently toxic cryoprotective agents (CPAs) or low temperature conditions, in bivalves. "
07/01/1989 - "The analysis illustrates the significance of perfusate osmolarity as a determinant of vascular resistance, can be used to investigate the hemodynamic effects that occur during the introduction and removal of cryoprotective agents, and is relevant to the interpretation of results obtained with hypertonic solutions in blood volume restoration after hypovolemic shock."

10/01/2020 - "Influence of cryoprotective agents on protein biosynthesis in Krebs-2 ascites carcinoma and wheat germ cell-free systems."
10/01/1971 - "Nucleic acids and acid-soluble derivatives released by isotonic solutions of cryoprotective agents from Ehrlich-Lettré ascites carcinoma and mouse spleen cells."
10/01/2020 - "The goal was to study the effect of penetrating cryoprotective agents glycerol, ME2SO, ethylene glycol, and non-penetrating PEG-400 on protein synthesizing activity in cell-free systems of Krebs-2 ascites carcinoma and wheat germ. "

10/01/2020 - "Influence of cryoprotective agents on protein biosynthesis in Krebs-2 ascites carcinoma and wheat germ cell-free systems."
10/01/1971 - "Nucleic acids and acid-soluble derivatives released by isotonic solutions of cryoprotective agents from Ehrlich-Lettré ascites carcinoma and mouse spleen cells."
10/01/2020 - "The goal was to study the effect of penetrating cryoprotective agents glycerol, ME2SO, ethylene glycol, and non-penetrating PEG-400 on protein synthesizing activity in cell-free systems of Krebs-2 ascites carcinoma and wheat germ. "

01/01/2022 - "Successful cryopreservation requires the addition of cryoprotective agents to protect against freezing damage and dehydration. "
09/01/2012 - "Freezing-induced cellular and membrane dehydration in the presence of cryoprotective agents."
07/01/1984 - "The present work shows that a combination of partial dehydration of blastomeres at room temperature with their permeation by a cryoprotective agent offers a simple method for successful rapid freezing and thawing of rabbit embryos."
02/02/2022 - "The correct cryopreservation procedure is characterized by the balance between freezing rate and cryoprotective agents (CPAs), which minimizes cellular dehydration and intracellular ice formation. "
10/30/2019 - "Use of heavy water allowed distinguishing water fluxes associated with dehydration and permeation, both of which were found to precede permeation of cryoprotective agents. "