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Aequorin

A photoprotein isolated from the bioluminescent jellyfish Aequorea. It emits visible light by an intramolecular reaction when a trace amount of calcium ion is added. The light-emitting moiety in the bioluminescence reaction is believed to be 2-amino-3-benzyl-5-(p-hydroxyphenyl)pyrazine (AF-350).
Also Known As:
Aequorine; Aequorins
Networked: 88 relevant articles (2 outcomes, 9 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1. Pieske, Burkert: 4 articles (05/2005 - 01/2002)
2. Furuichi, Takuya: 3 articles (01/2006 - 07/2005)
3. Lin, Cun: 2 articles (01/2006 - 07/2005)
4. Yoshizuka, Kazuharu: 2 articles (01/2006 - 07/2005)
5. Kadono, Takashi: 2 articles (01/2006 - 07/2005)
6. Kawano, Tomonori: 2 articles (01/2006 - 07/2005)
7. von Lewinski, Dirk: 2 articles (05/2005 - 02/2003)
8. Kögler, Harald: 2 articles (05/2005 - 02/2003)
9. El'-Registan, G I: 2 articles (11/2004 - 11/2004)
10. Kupriianova-Ashina, F G: 2 articles (11/2004 - 11/2004)

Related Diseases

1. Acidosis
2. Follicular Lymphoma (Lymphoma, Small Cleaved Cell, Follicular)
3. Shock
4. Infection
11/01/2002 - "On the negative side, this technique has also some disadvantages: (i) the relatively low amount of emitted light makes difficult performing single-cell imaging studies; (ii) reconstitution of aequorin with coelenterazine requires previous complete depletion of Ca(2+) of the ER for 1-2h, a maneuver that may result in deleterious effects in some cells; (iii) because of the high rate of aequorin consumption at steady-state [Ca(2+)](ER), only relatively brief experiments can be performed; and (iv) expression of ER-targeted aequorin requires previous transfection or infection to introduce the appropriate DNA construct, or alternatively the use of stable cell clones. "
11/01/2008 - "On the negative side, this technique has also some disadvantages: (i) the relatively low amount of emitted light makes difficult performing single-cell imaging studies; (ii) reconstitution of aequorin with coelenterazine is necessary to generate the functional photoprotein and this procedure requires at least 1h; (iii) in the case of aequorin targeted to high Ca2+ compartments, because of the high rate of aequorin consumption at steady-state, only relatively brief experiments can be performed and, because of the steepness of the Ca2+-response curve, the calibrated [Ca2+] values may not reflect the real mean in cells or compartments with dyshomogeneous behavior; and (iv) expression of targeted aequorins requires previous transfection or infection to introduce the appropriate DNA construct, or alternatively the use of stable cell clones."
5. Hypertrophy

Related Drugs and Biologics

1. Calcium
2. DNA (Deoxyribonucleic Acid)
3. Aequorin
4. coelenterazine
5. N-Methylaspartate (NMDA)
6. Saponins
7. Minocycline (Cyclops)
8. Egtazic Acid (EGTA)
9. Dimethyl Sulfoxide (DMSO)
10. 3-N-oxalyl-L -2,3-diaminopropanoic acid

Related Therapies and Procedures

1. Microelectrodes