calcein AM

a non-fluorescent compound cleaved to a fluorescent compound by non-specific intracellular esterases

Also Known As:

4',5'-bis(N,N-bis(carboxymethyl)aminomethyl)fluorescein acetoxymethyl ester; calcein-AM; calcein-acetoxymethyl ester; Glycine, N,N'-((3',6'-bis(acetyloxy)-3-oxospiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-diyl)bis(methylene))bis(N-(2-((acetyloxy)methoxy)-2-oxoethyl))-, bis((acetyloxy)methyl) ester

Networked: 111 relevant articles (2 outcomes, 11 trials/studies)

Relationship Network

Bio-Agent Context: Research Results

Experts

1.	Bystrzejewski, Michal: 3 articles (05/2014 - 01/2013)
2.	Cieszanowski, Andrzej: 3 articles (05/2014 - 01/2013)
3.	Cywinska, Monika A: 3 articles (05/2014 - 01/2013)
4.	Grudzinski, Ireneusz P: 3 articles (05/2014 - 01/2013)
5.	Kosmider, Anita: 3 articles (05/2014 - 01/2013)
6.	Ostrowska, Agnieszka: 3 articles (05/2014 - 01/2013)
7.	Poplawska, Magdalena: 3 articles (05/2014 - 01/2013)
8.	Wang, Wei: 2 articles (07/2021 - 01/2008)
9.	Wiese, Michael: 2 articles (04/2016 - 08/2009)
10.	Guo, Cheng: 2 articles (05/2014 - 12/2011)

Related Diseases

1.	Neoplasms (Cancer) 10/01/2022 - "The 3D tumor models were exposed to 0 to 8 Gy of radiation dose, and the radioresponse index (RTauc, area under the dose-response curve) was measured quantitatively with Calcein AM staining of live tumor cells. " 03/03/2022 - "Moreover, the efflux of Calcein AM, a well-known substrate for ABCB1, was also significantly impaired in BGJ 398-treated cancer cells, thereby suggesting the ABCB1 as a novel molecular target for BGJ 398. " 10/01/2021 - "In vitro cytotoxicity assay was performed with calcein AM release assay, and in vivo anti-tumor effect was compared using a U937 xenograft model. " 01/01/2021 - "The cancer cell death by FA-CD/PPy-NPs was confirmed by fluorescence imaging of FA-CDs as well as live/dead cell staining assay (calcein-AM/PI). " 01/01/2020 - "a tumor cell, based on calcein-AM staining and fluorescence detection. "
2.	Hyperthermia 01/01/2020 - "The efficacy of magnetic hyperthermia therapy was evaluated by using the cell viability test through the following fluorescence staining: calcein acetoxymethyl ester (492/513nm), for live cells, and ethidium homodimer-1 (526/619nm) for dead cells dyes. "
3.	Neuroblastoma 02/01/2000 - "Neuroblastoma cell lines (GD2-positive: SMS-KCN, SMS-LHN, LA-N-1; GD2-negative: SK-N-SH) were preloaded with calcein acetoxymethyl ester for the cytotoxicity assay or labeled in situ after 7 days of culture with fluorescein diacetate in the growth assay. " 06/01/2002 - "ADCC against LA-N-1 human neuroblastoma cells was evaluated with 4-hour calcein acetoxymethyl ester (calcein-AM) microcytotoxicity assay, electron microscopy, and multi-parameter flow cytometry. " 08/05/2009 - "After anti-GD2 conjugated CNTs were incubated with neuroblastoma cells for 6 h and endocytosed by the cells, CNT-laden neuroblastoma cells were further irradiated with an 808 nm near-infrared (NIR) laser with intensity ramping from 0.6 to 6 W cm(-2) for 10 min which was then maintained at 6 W cm(-2) for an additional 5 min. Post-NIR laser exposure, and after being examined by calcein-AM dye, stNB-V1 cells were all found to undergo necrosis, while non-GD2 expressing PC12 cells all remained viable. " 05/01/2000 - "This study compares the effective neuroprotective concentration of betaE2 against both A beta-mediated toxicity in a human neuroblastoma cell line, SK-N-SH using cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) as an endpoint to the effective betaE2 concentration obtained using a calcein acetoxymethyl ester (calcein AM) viability assay. " 03/01/2009 - "In human SH-SY5Y neuroblastoma cells, viability upon exposure to six LAs (bupivacaine, ropivacaine, mepivacaine, lidocaine, procaine, and chloroprocaine) was quantitatively determined by the MTT-(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetra-odium bromide) colorimetry assay and qualitatively confirmed by fluorescence imaging, using the LIVE/DEAD assay reagents (calcein/AM and ethidium homodimer-1). "
4.	Melanoma (Melanoma, Malignant) 09/01/1993 - "We compared initial arrest of murine B16F10 melanoma and D2A1 mammary carcinoma cells fluorescently labelled with calcein-AM, in low pressure (liver) vs high pressure (cremaster muscle) microvascular beds. " 05/05/2014 - "Meanwhile, the inhibitory effects of scutellarin on P-gp activity were examined on a human metastatic malignant melanoma cell line WM-266-4 by calcein-AM fluorometry screening assay. " 12/01/2011 - "The inhibitory effects of limonin on P-gp activity in a human metastatic malignant melanoma cell line WM-266-4 were examined using a calcein-AM fluorometry screening assay. " 01/01/2013 - "In this study, the cytotoxic effect of CEINs (the mean diameter distribution ranges 46-56 nm) has been explored by MTT, LDH leakage, Calcein-AM/propidium iodide (PI) and Annexin V-FITC/PI assays in human melanoma (HTB-140), mouse melanoma (B16-F10) cells, and human dermal fibroblasts (HDFs). " 11/28/2008 - "We treated glioma cell lines, LN-229 and SW1088, and melanoma cell lines, A375 and WM35, with two endothelin receptor type B (ETRB)-specific antagonists, A-192621 and BQ788, and quantified viable cells by the capacity of their intracellular esterases to convert non-fluorescent calcein AM into green-fluorescent calcein. "
5.	Hypoxia (Hypoxemia) 11/13/2023 - "In the in vitro study, calcein AM/PI staining and TUNEL staining showed that FX reduced cell death caused by hypoxia and reperfusion treatment. " 05/15/2022 - "Islets were treated with hypoxia for 24, 48, and 72 hours, Calcein-AM/PI staining showed that the number of dead cells gradually increased with time, which were significantly higher than those in the non-hypoxia-treated group ( P<0.001). " 05/15/2022 - "Calcein-AM/PI staining was used to evaluate the effect of COS on islet activity under hypoxia (1%O 2) conditions. " 04/01/2012 - "Cell viability (calcein AM assay) and expression of hypoxia-inducible factor-1α, EPO, Akt and Erk (immunoblot), and EPO receptor (reverse transcription-polymerase chain reaction) were analyzed. " 05/15/2022 - "Calcein-AM/PI staining indicated that GelMA/HAMA/COS hydrogels with different COS concentrations could improve islet viability under hypoxia conditions, and cell viability was positively correlated with COS concentration. "

Related Drugs and Biologics

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2.	ethidium homodimer
3.	calcein AM
4.	Propidium (Propidium Iodide)
5.	Member 1 Subfamily B ATP Binding Cassette Transporter
6.	Doxorubicin (Adriamycin)
7.	Paclitaxel (Taxol)
8.	fluorexon (calcein)
9.	Proteins (Proteins, Gene)
10.	FITC-annexin A5

Related Therapies and Procedures

1.	Therapeutics
2.	Lasers (Laser)
3.	Microspheres (Microsphere)