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calcein AM

a non-fluorescent compound cleaved to a fluorescent compound by non-specific intracellular esterases
Also Known As:
4',5'-bis(N,N-bis(carboxymethyl)aminomethyl)fluorescein acetoxymethyl ester; calcein-AM; calcein-acetoxymethyl ester; Glycine, N,N'-((3',6'-bis(acetyloxy)-3-oxospiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-diyl)bis(methylene))bis(N-(2-((acetyloxy)methoxy)-2-oxoethyl))-, bis((acetyloxy)methyl) ester
Networked: 63 relevant articles (0 outcomes, 8 trials/studies)

Bio-Agent Context: Research Results

Experts

1. Grudzinski, Ireneusz P: 2 articles (05/2014 - 04/2014)
2. Cieszanowski, Andrzej: 2 articles (05/2014 - 04/2014)
3. Ostrowska, Agnieszka: 2 articles (05/2014 - 04/2014)
4. Bystrzejewski, Michal: 2 articles (05/2014 - 04/2014)
5. Cywinska, Monika A: 2 articles (05/2014 - 04/2014)
6. Poplawska, Magdalena: 2 articles (05/2014 - 04/2014)
7. Kosmider, Anita: 2 articles (05/2014 - 04/2014)
8. Fijalek, Zbigniew: 2 articles (05/2014 - 04/2014)
9. Han, Yong-Long: 2 articles (01/2014 - 12/2011)
10. Guo, Cheng: 2 articles (01/2014 - 12/2011)

Related Diseases

1. Neoplasms (Cancer)
2. Neuroblastoma
02/01/2000 - "Neuroblastoma cell lines (GD2-positive: SMS-KCN, SMS-LHN, LA-N-1; GD2-negative: SK-N-SH) were preloaded with calcein acetoxymethyl ester for the cytotoxicity assay or labeled in situ after 7 days of culture with fluorescein diacetate in the growth assay. "
08/05/2009 - "After anti-GD2 conjugated CNTs were incubated with neuroblastoma cells for 6 h and endocytosed by the cells, CNT-laden neuroblastoma cells were further irradiated with an 808 nm near-infrared (NIR) laser with intensity ramping from 0.6 to 6 W cm(-2) for 10 min which was then maintained at 6 W cm(-2) for an additional 5 min. Post-NIR laser exposure, and after being examined by calcein-AM dye, stNB-V1 cells were all found to undergo necrosis, while non-GD2 expressing PC12 cells all remained viable. "
06/01/2002 - "ADCC against LA-N-1 human neuroblastoma cells was evaluated with 4-hour calcein acetoxymethyl ester (calcein-AM) microcytotoxicity assay, electron microscopy, and multi-parameter flow cytometry. "
05/01/2000 - "This study compares the effective neuroprotective concentration of betaE2 against both A beta-mediated toxicity in a human neuroblastoma cell line, SK-N-SH using cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) as an endpoint to the effective betaE2 concentration obtained using a calcein acetoxymethyl ester (calcein AM) viability assay. "
01/01/2005 - "In the present study, we exposed SHSY5Y neuroblastoma cells to neurotoxic beta amyloid peptides (Abeta) and oxygen glucose deprivation (OGD) to investigate the neuroprotective effect of 10 microM CoQ10 by measuring (i) cell viability by the MTT assay, (ii) opening of the mitochondrial permeability transition pore via the fluorescence intensity of calcein-AM, and (iii) superoxide anion concentration by hydroethidine. "
3. Leukemia
4. Ischemia
5. Necrosis
01/01/2011 - "Post-NIR laser exposure, when examined by calcein-AM dye, stNB-V1 cells all underwent necrosis, while non-GD2 expressing SH-SY5Y cells all remained viable. "
08/05/2009 - "After anti-GD2 conjugated CNTs were incubated with neuroblastoma cells for 6 h and endocytosed by the cells, CNT-laden neuroblastoma cells were further irradiated with an 808 nm near-infrared (NIR) laser with intensity ramping from 0.6 to 6 W cm(-2) for 10 min which was then maintained at 6 W cm(-2) for an additional 5 min. Post-NIR laser exposure, and after being examined by calcein-AM dye, stNB-V1 cells were all found to undergo necrosis, while non-GD2 expressing PC12 cells all remained viable. "
12/01/2007 - "With the SAC8.5 CCD camera and using DAPI, calcein-AM and propidium iodide we could also distinguish between viable, apoptotic and necrotic cells: exposure to CdCl(2) caused necrosis in A6 cells. "
10/01/2006 - "Cellular viability was examined with an ethidium homodimer and calcein-AM stain, whereas cellular apoptosis and/or necrosis were analyzed by a YO-PRO-1 dye/propidium iodide apoptosis assay coupled with flow cytometry and further confirmed with a genomic DNA pattern assay. "
01/01/2007 - "Cell death in response to OGD, alone or in combination with hydrogen peroxide, lipopolysaccharides, interleukin-1beta, tissue necrosis factor-alpha, or nitric oxide (NO) donor diethylenetriamine/nitric oxide adduct (DETA/NO) was quantified as the ratio of propidium iodide to calcein AM-positive cells. "

Related Drugs and Biologics

1. P-Glycoprotein
2. calcein AM
3. fluorexon (calcein)
4. Cyclosporine (Ciclosporin)
5. Verapamil (Calan)
6. L-Lactate Dehydrogenase (Lactate Dehydrogenase)
7. Rhodamine 123
8. Paclitaxel (Taxol)
9. Superoxides (Superoxide)
10. Peptides

Related Therapies and Procedures

1. Lasers (Laser)
2. Transplants (Transplant)