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3-O-methyl-fluorescein phosphatase

used for assay of Na-K-ATPase in intact tissues

Also Known As:

3-O-methylfluorescein phosphatase

Networked: 2 relevant articles (0 outcomes, 0 trials/studies)

Bio-Agent Context: Research Results

Enzymes and Coenzymes: 1
- Enzymes: 152586
  - Hydrolases: 2166
    - Acid Anhydride Hydrolases
      - Adenosine Triphosphatases: 8623
        P-type ATPases: 226
        Sodium-Potassium-Exchanging ATPase: 656
        3-O-methyl-fluorescein phosphatase: 2
Amino Acids, Peptides, and Proteins: 1
- Proteins: 484843
  - Carrier Proteins: 12999
    - Membrane Transport Proteins: 589
      - P-type ATPases: 226
        Sodium-Potassium-Exchanging ATPase: 656
        3-O-methyl-fluorescein phosphatase: 2
      - Ion Pumps: 61
        Antiporters: 369
        Sodium-Potassium-Exchanging ATPase: 656
        3-O-methyl-fluorescein phosphatase: 2
        Cation Transport Proteins: 22
        Sodium-Potassium-Exchanging ATPase: 656
        3-O-methyl-fluorescein phosphatase: 2
  - Membrane Proteins: 10078
    - Membrane Transport Proteins: 589
      - P-type ATPases: 226
        Sodium-Potassium-Exchanging ATPase: 656
        3-O-methyl-fluorescein phosphatase: 2
      - Ion Pumps: 61
        Antiporters: 369
        Sodium-Potassium-Exchanging ATPase: 656
        3-O-methyl-fluorescein phosphatase: 2
        Cation Transport Proteins: 22
        Sodium-Potassium-Exchanging ATPase: 656
        3-O-methyl-fluorescein phosphatase: 2

Experts

1.	Duhamel, T A: 1 article (07/2007)
2.	Foley, K P: 1 article (07/2007)
3.	Green, H J: 1 article (07/2007)
4.	Ouyang, J: 1 article (07/2007)
5.	Smith, I C: 1 article (07/2007)
6.	Stewart, R D: 1 article (07/2007)
7.	Aughey, R J: 1 article (07/2005)
8.	Cameron-Smith, D: 1 article (07/2005)
9.	Garnham, A P: 1 article (07/2005)
10.	Leppik, J A: 1 article (07/2005)

Related Diseases

1.

07/01/2007 - "Maximal Na(+)-K(+)-ATPase activity (V(max)) as measured by the 3-O-methylfluorescein phosphatase assay (nmol.mg(-1).h(-1)) was not different between conditions prior to exercise (85.2 +/- 3.3 or 86.0 +/- 3.9), at 30 min (91.4 +/- 4.7 vs. 91.9 +/- 4.1) and at fatigue (92.8 +/- 4.3 vs. 100 +/- 5.0) but was higher (P < 0.05) in G at 90 min (86.7 +/- 4.2 vs. 109 +/- 4.1). "
07/01/2005 - "A vastus lateralis muscle biopsy was taken at rest, fatigue, 3 h, and 24 h postexercise and analyzed for maximal Na(+)-K(+)-ATPase activity via 3-O-methylfluorescein phosphatase (3-O-MFPase) activity, Na(+)-K(+)-ATPase content via [(3)H]ouabain binding sites, and Na(+)-K(+)-ATPase alpha(1)-, alpha(2)-, alpha(3)-, beta(1)-, beta(2)- and beta(3)-isoform mRNA expression by real-time RT-PCR. "

Related Drugs and Biologics

1.	Adenosine Triphosphatases (ATPase)
2.	Messenger RNA (mRNA)
3.	Ouabain (G Strophanthin)