bis(1,3- diethylthiobarbiturate)trimethineoxonol

fluorescent probe for membrane potentials

Also Known As:

DiSBAC2; bis-(1,3-diethylthiobarbiturate) trimethine oxonol; bis-oxonol; 4,6(1H,5H)-Pyrimidinedione, 5-(3-(1,3-diethylhexahydro-4,6-dioxo-2-thioxo-5-pyrimidinyl)-2-propenylidene)-1,3-diethyldihydro-2-thioxo-

Networked: 13 relevant articles (0 outcomes, 0 trials/studies)

Bio-Agent Context: Research Results

Heterocyclic Compounds: 198
- 1-Ring Heterocyclic Compounds
  - Pyrimidines: 510
    - Pyrimidinones: 71
      - Barbiturates: 1074
        Thiobarbiturates: 4
        bis(1,3-diethylthiobarbiturate)trimethineoxonol: 13

Experts

1.	Botana, Luis M: 2 articles (01/2007 - 01/2006)
2.	Cagide, Eva: 2 articles (01/2007 - 01/2006)
3.	Louzao, M Carmen: 2 articles (01/2007 - 01/2006)
4.	Vieytes, Mercedes R: 2 articles (01/2007 - 01/2006)
5.	Yasumoto, Takeshi: 2 articles (01/2007 - 01/2006)
6.	Clementi, Emily A: 1 article (02/2014)
7.	Håkansson, Anders P: 1 article (02/2014)
8.	Marks, Laura R: 1 article (02/2014)
9.	Roche-Håkansson, Hazeline: 1 article (02/2014)
10.	Gao, Lijuan: 1 article (01/2013)

Related Diseases

1.	Neuroblastoma 01/01/2013 - "Neuroblastoma cells were incubated with the fluorescent dye bis-oxonol, whose distribution across the membrane was potential-dependent. " 01/01/2007 - "We studied the effects of a synthethic analog of polycavernoside A on human neuroblastoma cells by measuring changes in membrane potential with bis-oxonol and variations in intracellular calcium levels with fura-2. " 01/01/2006 - "We studied the effect of gambierol in human neuroblastoma cells by using bis-oxonol to measure membrane potential and FURA-2 to monitor intracellular calcium. "
2.	Leukemia 03/01/1987 - "Receptor-mediated changes in plasma membrane potential were recorded in rat basophilic leukemia (RBL) cells with the potential-sensitive fluorescent indicator bis-oxonol. " 09/25/1989 - "Changes in the plasma membrane potential of two histamine-releasing cells, rat peritoneal mast cells and basophilic leukemia cells 2H3 (RBL 2H3), were recorded with the potential-sensitive dye bis-oxonol. " 03/01/1987 - "IgE receptor-mediated depolarization of rat basophilic leukemia cells measured with the fluorescent probe bis-oxonol."
3.	Ischemia 12/01/2005 - "Endothelial cell fluorescence with bis-oxonol, a membrane potential probe, increased during lung ischemia indicating cell membrane depolarization. " 05/01/1998 - "In situ subpleural fluorescence microscopy showed that endothelial cells are the major site of the increased bis-oxonol fluorescence signal with ischemia. " 05/01/1998 - "Surface fluorometry showed increased bis-oxonol fluorescence (34.7 +/- 3.3% above baseline) and decreased JC-1 fluorescence (24.5 +/- 4.5% below baseline) with ischemia, compatible with membrane depolarization. "
4.	Rupture 02/17/2014 - "We developed protocols using the bis-oxonol dye DiBAC4(3) and the cell-impermeant dye propidium iodide to measure membrane depolarization and rupture, respectively, as well as methods to optimally load the pneumococci with the AM esters of the ratiometric dyes Fura-2, PBFI, and BCECF to detect changes in intracellular concentrations of Ca(2+), K(+), and H(+), respectively, using a fluorescence-detection plate reader. "
5.	Neoplasms (Cancer) 03/15/1997 - "Confocal laser scanning microscopy (CLSM) was employed to visualize and measure membrane potential changes in several types of cultured adherent cells, such as human fibroblasts, mouse mammary tumor C127 cells, and human saphenous vein endothelial cells, preloaded with the anionic dye bis-1, 3,-diethylthiobarbituratetrimethineoxonol (bis-oxonol). "

Related Drugs and Biologics

1.	Coloring Agents (Dyes)
2.	Fura-2 (Fura 2)
3.	Fluorescent Dyes (Fluorescent Probes)
4.	Indicators and Reagents (Reagents)
5.	Calcium
6.	5,5',6,6'- tetrachloro- 1,1',3,3'- tetraethylbenzimidazolocarbocyanine
7.	IgE Receptors (Fc epsilon RI)
8.	Veratridine
9.	Saponins
10.	Propidium (Propidium Iodide)

Related Therapies and Procedures

1.	Lasers (Laser)