thermitase

09/01/1992 - "Next, mutations and deletions derived from thermitase were introduced near the second autolysis loop in subtilisin BPN' (residues 158-165). "
09/01/1992 - "To improve the resistance to thermal-induced autolysis, we replaced sequences around these two sites with sequences derived from a thermophilic homologue of subtilisin, thermitase. "
01/01/1982 - "Ca2+-ions (20 mM) have a stabilizing effect against both autolysis and thermal denaturation (inactivation) of thermitase."
01/01/1982 - "Thermitase, the main component of the proteases of the culture medium from Thermoactinomyces vulgaris, is degraded by autolyses (increase of liberated amino groups) and thereby inactivated especially at elevated temperature, at alkaline pH-values and in the absence of added substrates. "
01/01/1982 - "As shown by polyacrylamide gel electrophoresis autolysis is an essential part during heat inactivation (complete disappearance of the thermitase band after heating the enzyme at 85 degrees C for 5 min). "