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cibacron blue-sepharose
affinity chromatography of alpha-2-macroglobulin
Also Known As:
Blue Sepharose CL-6B; Cibacron Blue Sepharose; cibacron blue F 3G-A sepharose; cibacron blue F3GA-agarose; cibacron blue agarose; cibracron blue-sepharose
Networked:
11
relevant articles (
0
outcomes,
0
trials/studies)
Bio-Agent Context: Research Results
Carbohydrates: 22023
Polysaccharides: 15383
Sepharose: 2476
cibacron blue-sepharose: 11
Related Diseases
1.
Experimental Liver Neoplasms
01/01/1993 - "
Comparison of pyruvate kinase variants from rat liver and Morris hepatoma 7777, obtained by an affinity chromatography on blue sepharose CL-6B.
"
01/01/1993 - "
Fractions A (salted out by ammonium sulphate between 21-30% saturation), and fractions B (salted out between 51-70% saturation) of pyruvate kinase (EC 2.7.1.40.) corresponding respectively to pyruvate kinase types L and M2 from rat liver and Morris hepatoma 7777 were purified by an affinity chromatography on Blue Sepharose CL-6B.
"
01/01/1998 - "
Cytosolic fractions B (salted out between 51-70% ammonium sulphate saturation) from rat liver and Morris hepatoma 7777, containing pyruvate kinase (EC 2.7.1.40) M2 isoenzymes, were purified by affinity chromatography on Blue Sepharose CL-6B.
"
01/04/1996 - "
Cytosolic pyruvate kinase fractions A and B obtained by salting out procedure from normal rat liver and Morris hepatoma 7777, purified by affinity chromatography on Blue Sepharose CL-6B, have shown similar electrophoretic patterns in polyacrylamide gel at pH 8.3 to previously studied pyruvate kinase extracts from chromatin of cell nuclei.
"
2.
Neoplasms (Cancer)
07/01/1980 - "
Different chromatographic behaviours on blue sepharose CL-6B of free and estradiol complexed forms of the estrogen receptor from DMBA-induced rat mammary tumors.
"
06/06/1988 - "
In order to analyse the incorporation of phosphate in the respective isozymes after endogenous phosphorylation of cytosolic extracts with [32P]ATP, homotetrameric isozymes as well as heterotetrameric hybrids of differentiated tumors were resolved by affinity chromatography on Blue-Sepharose CL-6B and, if necessary, further purified by immunoprecipitation.
"
3.
Small Cell Lung Carcinoma (Small Cell Lung Cancer)
08/01/1992 - "
An immunotoxin (IT) comprising abrin A chain attached to the mouse monoclonal antibody SWA11, recognising a cell surface antigen highly associated with human small cell lung cancer (SCLC), was synthesised using a hindered disulphide crosslinker, N-succinimidyl 3-(2-pyridyldithio) butyrate (SPDB), and purified by Blue Sepharose CL-6B affinity chromatography.
"
4.
Duchenne Muscular Dystrophy (Muscular Dystrophy, Becker)
01/01/1987 - "
Two isozymes of adenylate kinase from human Duchenne muscular dystrophy serum, one of which was an aberrant form specific to DMD patients, were separated by Blue Sepharose CL-6B affinity chromatography.
"
5.
Seminoma
02/01/1987 - "
An alkaline phosphatase (EC 3.1.3.1) of the placental type was isolated from a seminoma type of human testicular cancer tissue and was purified to homogeneity by sulfate-mediated chromatography on a column of Cibacron Blue Sepharose 4B.
"
Related Drugs and Biologics
1.
Pyruvate Kinase
2.
Isoenzymes
3.
Ammonium Compounds
4.
Octoxynol (Triton X 100)
5.
Sulfates (Sulfates, Inorganic)
6.
Salts
7.
Estrogen Receptors
8.
Poly I
9.
Poly C
10.
Phosphates (Orthophosphate)